Fig. 5

Morphological screening identifies CDM formulations with lower growth factor concentration without compromising phenotype or growth. (A) Full factorial design parameters for screening growth factor concentrations. (B) PCA plot representing morphological comparison between treatment groups and controls. (C) Table of final identified hits to be included in subsequent expansion where the value in parenthesis reflects percent change in concentration for each growth factor. (D) MSC proliferation of identified hits versus controls after 48 h of culture where the dashed line represents the initial seeding density. Cell count data from each treatment group was compared to SCM on a per-plate basis via ordinary one-way ANOVA corrected for multiple comparisons via Dunnett’s test. *p < 0.05 compared to SCM, #p < 0.05 compared to Hit G (+ CTL). n = 12 per plate for SCM, n = 12 per plate for CDBM, n = 8 per plate for Hit G (+ CTL). n = 4 for each treatment group. (E) Representative images from screen of MSCs grown in various media (red = actin, Golgi, plasma membrane, blue = nucleus, green = endoplasmic reticulum, magenta = mitochondria. Scale bar = 200 μm)